Detecção molecular de Hepatozoon canis e Babesia canis vogeli em cães domésticos de Cuiabá, Brasil

The objective of this study was to report for the first time infection by Hepatozoon spp. and Babesia spp. in 10 dogs from the city of Cuiabá, State of Mato Grosso, central-western Brazil. A pair of primers that amplifies a 574 bp fragment of the 18S rRNA of Hepatozoon spp., and a pair of primers that amplifies a 551 bp fragment of the gene 18S rRNA for Babesia spp. were used. Six dogs were positive for Babesia spp., and 9 were positive for Hepatozoon spp. Co-infection of Babesia spp. and Hepatozoon spp. was seen in 5 dogs. Sequenced samples revealed 100% identity with B. canis vogeli, and H. canis. This is the first molecular detection of H. canis in domestic dogs from Cuiabá. Additionally, it is described for the first time the presence of B. canis vogeli circulating among dogs in Cuiabá.O objetivo deste estudo foi relatar pela primeira vez a infecção por Hepatozoon spp. e Babesia spp. em cães domésticos provenientes da cidade de Cuiabá, estado de Mato Grosso. Foram utilizados pares de primers que amplificam um fragmento de 574 pb do gene 18S rRNA de Hepatozoon spp., e 551 pb do gene 18S rRNA para Babesia spp. Dos 10 cães amostrados, 6 apresentaram-se positivos para Babesia spp., e 9 foram positivos para Hepatozoon spp. pela PCR. Co-infecção entre Babesia spp. e Hepatozoon spp. ocorreu em 5 cães. As amostras revelaram 100% de identidade com B. canis vogeli, e as amostras que foram positivas para Hepatozoon spp. foram 100% idênticas a H. canis. Esta é a primeira identificação molecular de H. canis em cães domésticos em Cuiabá. Adicionalmente, descrevemos pela primeira vez a presença de B. canis vogeli circulando entre cães em Cuiabá.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Bilateral hemimelia of radio in canine – case report<br>Hemimelia bilateral de rádio em canino – relato de caso

Hemimelia or agenesis is a congenital morphological change that may affect the appendicular skeleton. This rare anomaly is the total or partial absence of one or more bones matched. It may present unilaterally or bilaterally, with the most frequent unilateral. In dogs, the most common form of the radial hemimelia is that causes deformity of the affected limb and severe functional limitations. Was Veterinary Hospital of the Federal University of Mato Grosso (HOVET/UFMT), a canine, female mongrel, with 30 days of age, presenting deformity in thoracic limbs since birth, observed signs of lameness and difficulty support. After complete bone development the animal returned for re-evaluation and establishment of appropriate treatment. On radiographic examination, there was bilateral agenesis of radios, angulation of the ulnar cranially, fusion of carpal bones proximal and distal to the ranks, bilateral scapulohumeral dislocation and the presence of a bone structure with well-defined boundaries and rectangular articulated with the scapula. Due to good quality of life of the animal and reserved prognosis of surgery, we opted for conservation of members. The owner was recommended to keep the animal in an environment with soft ground and castration.<p><p> Hemimelia ou agenesia é uma alteração morfológica congênita que pode acometer o esqueleto apendicular. Esta anomalia rara consiste na ausência total ou parcial de um ou mais ossos pareados. Pode se apresentar de forma uni ou bilateral, sendo a unilateral mais frequente. Em cães, a forma mais comum de hemimelia é a radial, que provoca deformidade do membro afetado e severas limitações funcionais. Foi atendido no Hospital Veterinário da Universidade Federal de Mato Grosso (HOVET/UFMT), um canino, fêmea, sem raça definida, com 30 dias de idade, apresentando deformidade nos membros torácicos desde o nascimento, claudicação e dificuldade de apoio. Após o completo desenvolvimento ósseo o animal retornou para uma reavaliação e estabelecimento do tratamento adequado. O exame radiográfico revelou agenesia bilateral dos rádios, angulação das ulnas no sentido cranial, fusão dos ossos cárpicos das fileiras proximal e distal, luxação escapuloumeral bilateral e presença de estrutura óssea com limites bem definidos e formato retangular articulada com as escápulas. Devido à boa qualidade de vida do animal e ao reservado prognóstico de uma intervenção cirúrgica, optou-se pelo tratamento conservativo, sendo recomendada a castração e ambiente de piso macio

YU Pyloroplasty for the Correction of Chronic Hypertrophic Pyloric Gastropathy in Canines

Background: One of the causes of delay gastric emptying is the chronic hypertrophic pyloric gastropathy, unusual disease that can occur in acquired or congenital form. It is characterized by hypertrophy of the antral mucosa, by thickening of the muscular layer, or by hyperplasia of the pylorus. The animals are presented with a history of chronic intermittent vomiting. Treatment is based on surgical correction through techniques such as pyloromyotomy or transverse or Y-U pyloroplasty. Thus, the aim was report three cases of pyloric stenosis by hypertrophy in dogs of the breeds, Fila Brasileiro, English Bull Terrier and American Pitbull Terrier, emphasizing the diagnosis and therapy instituted.Cases: All animals in this study reached the Veterinary Hospital of the Federal University of Mato Grosso with a history of chronic intermittent vomiting. The findings of physical and laboratory examinations in all animals were unspecific, with those important to rule out other causes of vomiting. Contrast radiographic evaluation revealed marked stomach distension and delayed gastric emptying, indicated gastric outflow obstruction. In one case, a video endoscopy was performed. The images reveal hyperemia of the gastric mucosa with small areas of bleeding around the stomach. It was observed intense production of gastric juice and the presence of the same reflux. In the pyloric antrum, a thick fold reddish gastric mucosa, which partially obstructed the pyloric ostium. There was very little motility during the examination. As therapeutic approach for all cases, we opted for performing the surgical technique of pyloroplasty in Y-U, consisting of enlarging the diameter of the antral pyloric region in an attempt to create a broader pylorus, what make possible an emptying a gastric rapidly. All the pacients remained in hospital in the postoperative period receiving medications intravenously. Postoperative fluid based on Ringer’s lactate solution and administration of ondansetron (0.2 mg/kg every 8 h), sucralfate (30 mg/ kg every 12 h), sulfadoxine to trimethoprim (20 mg/kg every 12 h) and meloxicam (0.2 mg/kg every 24 h). Twelve h of fasting after the surgical procedure without vomiting, the dog received light past food. An average of three to four days post-surgical discharged with recommendations to keep antibiotic therapy for ten days and light diet. The fifteenth day of surgery, the patients returned for removal of skin spots, with good general condition and satisfactory healing. No cases of vomiting were reported. As the case one returned to perform the monitoring gastograma. The dog was anesthetized and giving the contrast. On radiographs, there was a slight delay in gastric emptying, which can be explained by the anesthesia used for contrast administration, as described in the literature. The images showed stomach and intestines in size, shape and normal position. The technique was satisfactory in reducing clinical signs in all these cases, confirmed by the absence of vomiting in the post-operative period.Discussion: The surgical technique used, pyloroplasty Y-U consists of traspor a part of the antral wall to the pyloric region in an attempt to create a broader pylorus, and possibly a time soon gastric emptying. It was possible to demonstrate with only three cases the pyloroplasty Y-U proved to be a simple and effective technique for the treatment of hypertrophic pyloric stenosis with a favorable prognosis in all cases. Thus, disease should be considered in the differential diagnosis in cases of gastric outflow obstruction, regardless of patients age.Keywords: dog, hypertrophy, pylorus